Membrane estrogen receptors stimulate intracellular calcium release and progesterone synthesis in hypothalamic astrocytes.
Identifieur interne : 002623 ( Main/Exploration ); précédent : 002622; suivant : 002624Membrane estrogen receptors stimulate intracellular calcium release and progesterone synthesis in hypothalamic astrocytes.
Auteurs : John Kuo [États-Unis] ; Naheed Hamid ; Galyna Bondar ; Eric R. Prossnitz ; Paul MicevychSource :
- The Journal of neuroscience : the official journal of the Society for Neuroscience [ 1529-2401 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Astrocytes (métabolisme), Calcium (métabolisme), Calcium (physiologie), Cellules cultivées, Femelle, Hypothalamus (cytologie), Hypothalamus (métabolisme), Liquide intracellulaire (métabolisme), Membrane cellulaire (métabolisme), Membrane cellulaire (physiologie), Oestradiol (physiologie), Progestérone (biosynthèse), Rat Long-Evans, Rats, Récepteur alpha des oestrogènes (agonistes), Récepteur alpha des oestrogènes (déficit), Récepteur alpha des oestrogènes (génétique), Récepteur alpha des oestrogènes (physiologie), Signalisation du calcium (physiologie), Souris, Souris de lignée C57BL, Souris knockout.
- MESH :
- agonistes : Récepteur alpha des oestrogènes.
- biosynthèse : Progestérone.
- cytologie : Hypothalamus.
- déficit : Récepteur alpha des oestrogènes.
- génétique : Récepteur alpha des oestrogènes.
- métabolisme : Astrocytes, Calcium, Hypothalamus, Liquide intracellulaire, Membrane cellulaire.
- physiologie : Calcium, Membrane cellulaire, Oestradiol, Récepteur alpha des oestrogènes, Signalisation du calcium.
- Animaux, Cellules cultivées, Femelle, Rat Long-Evans, Rats, Souris, Souris de lignée C57BL, Souris knockout.
English descriptors
- KwdEn :
- Animals, Astrocytes (metabolism), Calcium (metabolism), Calcium (physiology), Calcium Signaling (physiology), Cell Membrane (metabolism), Cell Membrane (physiology), Cells, Cultured, Estradiol (physiology), Estrogen Receptor alpha (agonists), Estrogen Receptor alpha (deficiency), Estrogen Receptor alpha (genetics), Estrogen Receptor alpha (physiology), Female, Hypothalamus (cytology), Hypothalamus (metabolism), Intracellular Fluid (metabolism), Mice, Mice, Inbred C57BL, Mice, Knockout, Progesterone (biosynthesis), Rats, Rats, Long-Evans.
- MESH :
- chemical , agonists : Estrogen Receptor alpha.
- chemical , biosynthesis : Progesterone.
- chemical , deficiency : Estrogen Receptor alpha.
- chemical , genetics : Estrogen Receptor alpha.
- chemical , metabolism : Calcium.
- cytology : Hypothalamus.
- metabolism : Astrocytes, Cell Membrane, Hypothalamus, Intracellular Fluid.
- chemical , physiology : Calcium, Calcium Signaling, Cell Membrane, Estradiol, Estrogen Receptor alpha.
- Animals, Cells, Cultured, Female, Mice, Mice, Inbred C57BL, Mice, Knockout, Rats, Rats, Long-Evans.
Abstract
In hypothalamic astrocytes obtained from adult female rats, estradiol rapidly increased free cytoplasmic calcium concentrations ([Ca(2+)](i)) that facilitate progesterone synthesis. The present study demonstrated that estradiol (1 nm) significantly and maximally stimulated progesterone synthesis within 5 min, supporting a rapid, nongenomic mechanism. The group I metabotropic glutamate receptor (mGluR1a) antagonist LY 367385 [(S)-(+)-a-amino-4-carboxy-2-methylbenzeneacetic acid] attenuated both the estradiol-induced [Ca(2+)](i) release and progesterone synthesis. To investigate membrane-associated estrogen receptors (mERs), agonists for ERα, ERβ, STX-activated protein, and GPR30 were compared. The selective ERα agonist propylpyrazole triole (PPT) and STX most closely mimicked the estradiol-induced [Ca(2+)](i) responses, where PPT was more potent but less efficacious than STX. Only high doses (100 nm) of selective ERβ agonist diarylpropionitrile (DPN) and GPR30 agonist G-1 induced estradiol-like [Ca(2+)](i) responses. With the exception of DPN (even at 100 nm), all agonists stimulated progesterone synthesis. The PPT- and STX-induced [Ca(2+)](i) release and progesterone synthesis were blocked by LY 367385. While the G-1-stimulated [Ca(2+)](i) release was blocked by LY 367385, progesterone synthesis was not. Since GPR30 was detected intracellularly but not in the membrane, we interpreted these results to suggest that G-1 could activate mGluR1a on the membrane and GPR30 on the smooth endoplasmic reticulum to release intracellular calcium. Although STX and G-1 maximally stimulated [Ca(2+)](i) release in astrocytes from estrogen receptor-α knock-out (ERKO) mice, estradiol in vivo did not stimulate progesterone synthesis in the ERKO mice. Together, these results indicate that mERα is mainly responsible for the rapid, membrane-initiated estradiol-signaling that leads to progesterone synthesis in hypothalamic astrocytes.
DOI: 10.1523/JNEUROSCI.1158-10.2010
PubMed: 20881113
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001F32
- to stream PubMed, to step Curation: 001F32
- to stream PubMed, to step Checkpoint: 001E32
- to stream Ncbi, to step Merge: 000779
- to stream Ncbi, to step Curation: 000779
- to stream Ncbi, to step Checkpoint: 000779
- to stream Main, to step Merge: 002648
- to stream Main, to step Curation: 002623
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Membrane estrogen receptors stimulate intracellular calcium release and progesterone synthesis in hypothalamic astrocytes.</title><author><name sortKey="Kuo, John" sort="Kuo, John" uniqKey="Kuo J" first="John" last="Kuo">John Kuo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Neurobiology, Laboratory of Neuroendocrinology of the Brain Research Institute, Department of Obstetrics and Gynecology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Neurobiology, Laboratory of Neuroendocrinology of the Brain Research Institute, Department of Obstetrics and Gynecology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095</wicri:regionArea><wicri:noRegion>California 90095</wicri:noRegion></affiliation></author><author><name sortKey="Hamid, Naheed" sort="Hamid, Naheed" uniqKey="Hamid N" first="Naheed" last="Hamid">Naheed Hamid</name></author><author><name sortKey="Bondar, Galyna" sort="Bondar, Galyna" uniqKey="Bondar G" first="Galyna" last="Bondar">Galyna Bondar</name></author><author><name sortKey="Prossnitz, Eric R" sort="Prossnitz, Eric R" uniqKey="Prossnitz E" first="Eric R" last="Prossnitz">Eric R. Prossnitz</name></author><author><name sortKey="Micevych, Paul" sort="Micevych, Paul" uniqKey="Micevych P" first="Paul" last="Micevych">Paul Micevych</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20881113</idno><idno type="pmid">20881113</idno><idno type="doi">10.1523/JNEUROSCI.1158-10.2010</idno><idno type="wicri:Area/PubMed/Corpus">001F32</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001F32</idno><idno type="wicri:Area/PubMed/Curation">001F32</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001F32</idno><idno type="wicri:Area/PubMed/Checkpoint">001E32</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001E32</idno><idno type="wicri:Area/Ncbi/Merge">000779</idno><idno type="wicri:Area/Ncbi/Curation">000779</idno><idno type="wicri:Area/Ncbi/Checkpoint">000779</idno><idno type="wicri:Area/Main/Merge">002648</idno><idno type="wicri:Area/Main/Curation">002623</idno><idno type="wicri:Area/Main/Exploration">002623</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Membrane estrogen receptors stimulate intracellular calcium release and progesterone synthesis in hypothalamic astrocytes.</title><author><name sortKey="Kuo, John" sort="Kuo, John" uniqKey="Kuo J" first="John" last="Kuo">John Kuo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Neurobiology, Laboratory of Neuroendocrinology of the Brain Research Institute, Department of Obstetrics and Gynecology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Neurobiology, Laboratory of Neuroendocrinology of the Brain Research Institute, Department of Obstetrics and Gynecology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095</wicri:regionArea><wicri:noRegion>California 90095</wicri:noRegion></affiliation></author><author><name sortKey="Hamid, Naheed" sort="Hamid, Naheed" uniqKey="Hamid N" first="Naheed" last="Hamid">Naheed Hamid</name></author><author><name sortKey="Bondar, Galyna" sort="Bondar, Galyna" uniqKey="Bondar G" first="Galyna" last="Bondar">Galyna Bondar</name></author><author><name sortKey="Prossnitz, Eric R" sort="Prossnitz, Eric R" uniqKey="Prossnitz E" first="Eric R" last="Prossnitz">Eric R. Prossnitz</name></author><author><name sortKey="Micevych, Paul" sort="Micevych, Paul" uniqKey="Micevych P" first="Paul" last="Micevych">Paul Micevych</name></author></analytic><series><title level="j">The Journal of neuroscience : the official journal of the Society for Neuroscience</title><idno type="eISSN">1529-2401</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Astrocytes (metabolism)</term><term>Calcium (metabolism)</term><term>Calcium (physiology)</term><term>Calcium Signaling (physiology)</term><term>Cell Membrane (metabolism)</term><term>Cell Membrane (physiology)</term><term>Cells, Cultured</term><term>Estradiol (physiology)</term><term>Estrogen Receptor alpha (agonists)</term><term>Estrogen Receptor alpha (deficiency)</term><term>Estrogen Receptor alpha (genetics)</term><term>Estrogen Receptor alpha (physiology)</term><term>Female</term><term>Hypothalamus (cytology)</term><term>Hypothalamus (metabolism)</term><term>Intracellular Fluid (metabolism)</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term><term>Progesterone (biosynthesis)</term><term>Rats</term><term>Rats, Long-Evans</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Astrocytes (métabolisme)</term><term>Calcium (métabolisme)</term><term>Calcium (physiologie)</term><term>Cellules cultivées</term><term>Femelle</term><term>Hypothalamus (cytologie)</term><term>Hypothalamus (métabolisme)</term><term>Liquide intracellulaire (métabolisme)</term><term>Membrane cellulaire (métabolisme)</term><term>Membrane cellulaire (physiologie)</term><term>Oestradiol (physiologie)</term><term>Progestérone (biosynthèse)</term><term>Rat Long-Evans</term><term>Rats</term><term>Récepteur alpha des oestrogènes (agonistes)</term><term>Récepteur alpha des oestrogènes (déficit)</term><term>Récepteur alpha des oestrogènes (génétique)</term><term>Récepteur alpha des oestrogènes (physiologie)</term><term>Signalisation du calcium (physiologie)</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Souris knockout</term></keywords><keywords scheme="MESH" type="chemical" qualifier="agonists" xml:lang="en"><term>Estrogen Receptor alpha</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Progesterone</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Estrogen Receptor alpha</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Estrogen Receptor alpha</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term></keywords><keywords scheme="MESH" qualifier="agonistes" xml:lang="fr"><term>Récepteur alpha des oestrogènes</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Progestérone</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Hypothalamus</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Hypothalamus</term></keywords><keywords scheme="MESH" qualifier="déficit" xml:lang="fr"><term>Récepteur alpha des oestrogènes</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Récepteur alpha des oestrogènes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Astrocytes</term><term>Cell Membrane</term><term>Hypothalamus</term><term>Intracellular Fluid</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Astrocytes</term><term>Calcium</term><term>Hypothalamus</term><term>Liquide intracellulaire</term><term>Membrane cellulaire</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Calcium</term><term>Membrane cellulaire</term><term>Oestradiol</term><term>Récepteur alpha des oestrogènes</term><term>Signalisation du calcium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Calcium</term><term>Calcium Signaling</term><term>Cell Membrane</term><term>Estradiol</term><term>Estrogen Receptor alpha</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Female</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term><term>Rats</term><term>Rats, Long-Evans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Femelle</term><term>Rat Long-Evans</term><term>Rats</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Souris knockout</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In hypothalamic astrocytes obtained from adult female rats, estradiol rapidly increased free cytoplasmic calcium concentrations ([Ca(2+)](i)) that facilitate progesterone synthesis. The present study demonstrated that estradiol (1 nm) significantly and maximally stimulated progesterone synthesis within 5 min, supporting a rapid, nongenomic mechanism. The group I metabotropic glutamate receptor (mGluR1a) antagonist LY 367385 [(S)-(+)-a-amino-4-carboxy-2-methylbenzeneacetic acid] attenuated both the estradiol-induced [Ca(2+)](i) release and progesterone synthesis. To investigate membrane-associated estrogen receptors (mERs), agonists for ERα, ERβ, STX-activated protein, and GPR30 were compared. The selective ERα agonist propylpyrazole triole (PPT) and STX most closely mimicked the estradiol-induced [Ca(2+)](i) responses, where PPT was more potent but less efficacious than STX. Only high doses (100 nm) of selective ERβ agonist diarylpropionitrile (DPN) and GPR30 agonist G-1 induced estradiol-like [Ca(2+)](i) responses. With the exception of DPN (even at 100 nm), all agonists stimulated progesterone synthesis. The PPT- and STX-induced [Ca(2+)](i) release and progesterone synthesis were blocked by LY 367385. While the G-1-stimulated [Ca(2+)](i) release was blocked by LY 367385, progesterone synthesis was not. Since GPR30 was detected intracellularly but not in the membrane, we interpreted these results to suggest that G-1 could activate mGluR1a on the membrane and GPR30 on the smooth endoplasmic reticulum to release intracellular calcium. Although STX and G-1 maximally stimulated [Ca(2+)](i) release in astrocytes from estrogen receptor-α knock-out (ERKO) mice, estradiol in vivo did not stimulate progesterone synthesis in the ERKO mice. Together, these results indicate that mERα is mainly responsible for the rapid, membrane-initiated estradiol-signaling that leads to progesterone synthesis in hypothalamic astrocytes.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Bondar, Galyna" sort="Bondar, Galyna" uniqKey="Bondar G" first="Galyna" last="Bondar">Galyna Bondar</name><name sortKey="Hamid, Naheed" sort="Hamid, Naheed" uniqKey="Hamid N" first="Naheed" last="Hamid">Naheed Hamid</name><name sortKey="Micevych, Paul" sort="Micevych, Paul" uniqKey="Micevych P" first="Paul" last="Micevych">Paul Micevych</name><name sortKey="Prossnitz, Eric R" sort="Prossnitz, Eric R" uniqKey="Prossnitz E" first="Eric R" last="Prossnitz">Eric R. Prossnitz</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Kuo, John" sort="Kuo, John" uniqKey="Kuo J" first="John" last="Kuo">John Kuo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002623 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002623 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20881113
|texte= Membrane estrogen receptors stimulate intracellular calcium release and progesterone synthesis in hypothalamic astrocytes.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20881113" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |